Centrifuge Ultracentrifuge Preparative

Description

Samples are centrifuged in cells which are transparent top and bottom. A laser scans the cells at regular intervals during centrifugation, recording changes in absorbance along the cell. A typical result from a sedimentation velocity experiment is shown at top right above. Early scans (in blue) show the boundary moving as molecules start to sediment; later scans (in red) show the molecules have been cleared from the top of the cell, giving a low absorbance, and accumulating at the bottom of the cell. Larger molecules (e.g. multimers) will tend to sediment faster than smaller ones; globular, folded molecules will sediment faster than asymmetric or unfolded molecules of the same molecular weight. Different rotor speeds are chosen for analysis of different sized molecules.

Details

Manufacturer BECKMAN COULTER
Model No. OPTIMA L-80XP
Serial No. LXP03D11
Location Laboratory Centrifuge [1-086-0065-06FL-110E]
Identifier 10005149
Organisation: School of Biomedical Sciences [50025931]
Owner
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Category / Taxonomy Process Equipment - Biological > Centrifuge > Ultracentrifuges